One of Raphael's most talented assistants, Giulio Romano left Rome in 1524 to work for Federigo Gonzaga, ruler of Mantua. Romano's paintings contributed to the spread of Roman mannerism, a style which set the artistic standard for the next two decades in Italy. This painting illustrates the sophisticated and obscure symbols that were familiar to the courts of sixteenth-century Italy; to decipher the meanings of the various segments was a challenging game for courtiers. The most easily understood symbols are the phoenix rising out of the flames (a symbol of rebirth) and the serpent devouring its own tail (representing eternity).